Anti-α-enolase antibody combined with β2 microglobulin evaluated the incidence of nephritis in systemic lupus erythematosus patients
Y Huang, L Chen, K Chen, F Huang, Y Feng, Z Xu and W Wang
Department of Laboratory Medicine, Sun Yat-sen University affiliated Zhongshan Hospital, Zhongshan, China; and Department of Pharmacy,Shenzhen Children's Hospital, Shenzhen, China
Objective: Anti-α-enolase antibody (Ab) combined with β2 microglobulin (β2-MG) were investigated to predict the incidence of nephritis in systemic lupus erythematosus (SLE) patients. Methods: Levels of serum anti-β-enolase Ab and urinary β2-MG were detected in 115 SLE patients, 29 SLE patients with nephritis and 70 healthy controls by ELISA and immunoturbidimetry, respectively. Furthermore, the correlation between anti-α-enolase Ab combined with β2-MG and the incidence of nephritis in SLE patients was evaluated by correlation analysis. Results: The optical density value of serum anti-α-enolase Ab in SLE patients with nephritis (0.84) was greatly increased compared with SLE patients (0.76) or healthy controls (0.54). Moreover, the levels of urinary β2-MG in SLE patients with nephritis (6.75 mg/L) were increased compared with SLE patients (3.45 mg/L) or healthy controls (1.48 mg/L). There was a positive correlation between the level of anti-α-enolase Ab and β2-MG (r ¼ 0.754). Furthermore, anti-α-enolase Ab combined with β2-MG for evaluating the incidence of nephritis in SLE patients had the best assessment of the effectiveness (areaunder the receiver operating characteristic curve (AUC): 92.7%) compared with only anti-α-enolase Ab (AUC: 80.9%) or β2-MG (AUC: 84.5%). Conclusion: These data suggested that anti-α-enolase Ab may be a potential indicator for the prediction of nephritis in SLE patients. Lupus (2019) 28, 365-370.
The serum obtained from 144 SLE patients and 70 healthy controls were subjected to ELISA (#JL46123, Jianglai Biological, China), according to the manufacturer's instructions. Briefly, the Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of anti-α-enolase Ab in the sample, this anti-α-enolase Ab ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density (OD) versus anti-α-enolase Ab concentration. The concentration of anti-α-enolase Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Figure 1 Renal function indicators serum uric acid (UA) (a), cystatin C (CysC) (b), creatinine (Cr) (c) and urea (d) in healthy controls, systemic lupus erythematosus (SLE) patients and lupus nephritis (LN) patients.
Figure 2 Urinary β2 microglobulin and serum anti-α-enolase antibody in health controls, systemic lupus erythematosus (SLE) patients and lupus nephritis (LN) patients.
Figure 3 Receiver operating characteristic (ROC) curve for evaluating systemic lupus erythematosus complicated with nephritis by anti-α-enolase antibody (Ab) and β2 microglobulin (β2-MG).
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